Thanks benben. From this URL, there is a link to this
From there click on additional data file. Which brings you to MJA2-9999-na-s001.pdf.
From the pdf, we finally get to see how they "isolated".
Short answer: they never isolated.
Long answer: African green monkey kidney cells were grown in Earle’s minimum essential medium (EMEM), fetal serum, glutamine, sodium pyruvate, and sodium bicarbonate.
Then given the snot from the sick person.
They really call this process isolate
s. The virus was never isolated.
Notice, they used it on green monkey kidney cells. Not on human cells. They did test on human cells but nothing happened.
Another thing to notice about their experiment.
1. There was NO control group.
2. They only tested on 1 person.
If it was scientifically proven, you can duplicate the test on many people.
I don't agree with Koch's postulates. But aside, how would you know what to isolate to begin with? Given two groups, one sick and the other healthy, there will certainly be many differences between them. That's why experiments are necessary.
Virologist don't use Koch's postulates or River postulates. But if you say this is what causes these people sick, they need to prove it. My guess the reason why they don't use the postulates is because they can never get it to work. And this steps into another topic. Germ theory vs. Terrain theory. Germ theory has never been proven, yet that is exactly what vaccines are.
You can't sequence something if you don't isolate it. Otherwise you might sequence some other thing or things.
You can read what machine they used for sequencing.
Sequencing libraries were prepared using 300ng cDNA as input for the SQK-LSK109 kit (Oxford Nanopore Technologies, Oxford, UK) as per manufacturer’s instructions.